Specialized cell culture
Specialized cell culture/ Cells co-culture, cell-to-cell interaction, activation, and measurement of drug effect using immunofluorescence studies
ANALYSING IMMUNE CELL RESPONSE BY IN VITRO STIMULATION
Quantification of functional melanoma antigen (Melan-A/MART- 1) specific T-cell response using dual IFN-γ and TNF-α intracellular staining. T-cells were incubated with dendritic cells (DCs) pulsed with increasing amount of Melan-A/MART- 1 peptide. IFN-γ and TNF-α produced by Melan-A- MART-1- specific T cells were quantified by flow cytometry after gating on CD8+ T-cells.
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